Community structure and diversity of endophytic bacteria in tobacco variety Zhongchuan 208
[Objective]Zhongchuan 208 is a novel flue-cured tobacco variety with good quality,economic prospects,and disease and stress resistance,which is predominantly grown in Southwest and central parts of China.This study aims to investigate the bacterial community structure and species diversity constituents in Zhongchuan 208's four organs:namely,roots and above ground parts(stem,leaf and flower),and establish a basis of tobacco microbiome,in order to provide a knowledge base of exploiting and utilizing endophytic bacterial resources.[Methods]The aforementioned parts of Zhongchuan 208 were used as research materials,and their high-throughput amplicon sequencing of V3-V4 fragment of bacterial 16S rRNA genes was conducted using the Illumina NovaSeq 6000 platform.The bacterial community structure and species diversity of different organs as well as the effects of plant nutrition on bacterial community were examined using bioinformatics.[Results](l)There were 5460 amplicon sequence variants(ASVs)obtained from four samples of Zhongchuan 208,belonging to 19 phyla,72 classes,161 orders,285 families and 389 genera.(2)The bacterial community diversity of different organs could be ranked in the order of root＞stem≥flower＞leaf.The Beta diversity analysis and clustering analysis indicated a high degree of similarity in the community composition of endophytic bacteria between root and leaf of Zhongchuan 208,but a significant difference was observed in that between flower and stem.(3)Proteobacteria and Actinobacteria were the dominant phyla of endophytic bacterial communities in roots,stems and leaves,while Firmicutes and Bacteroidetes had the highest relative abundance in flowers.LEfSe analysis showed that the bacterial community composition of Zhongchuan 208 was significantly different at the genus level,the bacterial community was mainly characterized by different endophytic bacteria such as Acinetobacter,Brevundimonas,Allorhizobium,Bacillus,Chryseobacterium,Enterobacter,Alistipes,Lachnospira,Faecalibacterium,Ruminococcaceae_UCG_014 and Rosenbergiella in stem,leaf and flower.(4)Redundancy analysis showed that the contents of total N(R2=0.730,P＜0.001),total sugar(R2=0.883,P＜0.001)and Mg(R2=0.907,P＜0.01)in Zhongchuan 208 were strongly associated with the dominant bacterial communities found in these organs.[Conclusion]The bacterial community diversity in Zhongchuan 208 decreased from roots to flowers,stem and/or leaves,and the species composition of endophytic bacterial communities in different organs was significantly different.There are many significantly enriched bacterial flora in plant organs of Zhongchuan 208,which are worthy of further research and exploitation.
国家科技期刊平台
NSTL
万方数据
维普
