[目的]为探索烟草炭疽菌响应贝莱斯芽孢杆菌(Bacillus velezensis)F85拮抗的分子机制.[方法]以引起烟草炭疽病的Colletotrichum fructicola为研究对象,设置经F85无菌发酵液处理后的C.fructicola菌株(T)和正常生长的C.fructicola菌株(CK),利用转录组测序探究贝莱斯芽孢杆菌拮抗条件下烟草炭疽菌菌丝的差异表达基因(Differential Expressed Genes,DEGs)和代谢通路富集情况.[结果]B.velezensis无菌发酵液处理的C.fructicola菌株(T)与对照(CK)相比,共有2870个DEGs,其中1524个基因上调表达,1346个基因下调表达.KEGG富集分析结果表明,DEGs主要富集在苯丙氨酸代谢(Phenylalanine metabolism)、缬氨酸、亮氨酸和异亮氨酸降解(Valine,leucine and isoleucine degradation)等通路上,编码过氧化物酶体(KatGs)、醛脱氢酶(ALDH)、甲基丙二酸半醛脱氢酶(MoMsdh)等的基因显著下调表达.[结论]在贝莱斯芽孢杆菌F85拮抗下,烟草炭疽菌菌丝转录组特征发生显著变化,F85主要影响C.fructicola菌丝过氧化物酶体调控以及菌丝能量形成过程等.
Transcriptomic analysis of Bacillus velezensis F85 antagonizing Colletotrichum fructicola
[Background]This study aims to explore the molecular mechanism of Colletotrichum spp.in response to the antagonism effect of Bacillus velezensis F85.[Methods]Taking Colletotrichum fructicola,which causes tobacco anthracnose,as the research object,we set up a C.fructicola strain(T)treated with Bacillus velezensis F85 sterile fermentation broth and a normally growing C.fructicola strain(CK).We used transcriptome sequencing to explore the differentially expressed genes(DEGs)and the enrichment of metabolic pathways in the mycelium of tobacco anthracnose fungus under the antagonistic conditions of Bacillus velezensis..[Results]2870 DEGs were found between CK and T,including 1524 up-regulated genes and 1346 down-regulated genes.KEGG enrichment analysis showed that some pathways such as Phenylalanine metabolism,Valine,leucine and isoleucine degradation were significantly enriched by DEGs.Some genes coding KatGs,ALDH,CuAO,MoMsdh were down-expressed significantly.[Conclusion]Under the antagonism of B.velezensis F85,the characteristics of C.fructicola hyphae at transcriptomes level were significantly changed,and the B.Velesiensis F85 significantly affected the regulation of peroxisome and energy formation process of C.fructicola mycelium.
Bacillus velezensisColletotrichum fructicolatranscriptome analysisbiological control
国家科技期刊平台
NSTL
万方数据
维普
