Differential analysis of metabolome of cigar tobacco leaves after curing in typical production areas of Hainan
[Background]This study aims to explore the chemical basis of the quality difference of the variety"Haiyan 201"cigar leaves after curing in two typical production areas in Hainan.[Methods]Based on high-performance liquid chromatography tandem mass spectrometry(HPLC-MS/MS)non-targeted metabolomics approach,the study analyzed the differences in metabolites and pathways in cigar tobacco leaves of"Haiyan201"after curing in Wuzhishan Fanyang and Danzhou Guangcun,two typical production areas.[Results](1)H Five main tobacco production areas in Hainan can be divided into two categories:Wuzhishan Fanyang,Changjiang Haiwei,and Baisha Rongbang as one category,and Danzhou Guangcun and Dongfang Datian as another.(2)There were significant differences in the relative contents of 222 metabolites in air-cured cigar leaves from Wuzhishan Fanyang and Danzhou Guangcun.(3)KEGG database annotation and enrichment results show that the differential metabolites in Wuzhishan Fanyang and Danzhou Guangcun regions are mainly enriched in biosynthesis of amino acids pathway,metabolic pathway and arginine biosynthesis pathway.The biosynthetic pathway of amino acids shows the most significant difference.The relative contents of L-yeastamine,L-valine,L-glutamine,L-citrulline,S-adenosylmethionine,N-acetyl-DL-glutamine are significantly lower in Danzhou Guangcun than in Wuzhishan Fanyang.(4)Sensory quality evaluation shows that cigar tobacco leaves from Danzhou Guangcun after curing are superior in aroma richness,odor,and quality characteristics compared to those from Wuzhishan Fanyang.[Conclusion]There were significant differences in metabolic spectra of cigar leaves between the two typical producing areas,especially in the contents of some free amino acids and their derivatives,which may be related to the quality of tobacco leaves in these areas.The results of this study will provide theoretical support for subsequent technical improvements and characteristic enhancement of cigar raw materials.
cigar curingmetabolomicsregional differencebiosynthesis of amino acids
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