Identification of vitellogenin gene in Ephestia elutella and its response to X-ray irradiation stress
[Background]Vitellogenin(Vg)is the precursor protein of egg yolk vitellin(Vn)in insects.This study aims to explore the effects of X-ray irradiation stress on the transcript expression of Vg gene and fecundity of tobacco moth Ephestia elutella,in order to lay the foundation for revealing the molecular mechanism.[Methods]The full length of the EeVg gene was cloned by RT-PCR and analyzed by bioinformatics methods.RT-qPCR technology was used to detect the expression patterns of the gene in different tissues(foot,head,midgut,fat body,ovary and epidermis).Female pupae of E.elutella were irradiated via an X-ray irradiator X-RAD 320.Then the effects of X-ray irradiation at the doses of 50,100 and 150 Gy on the emergence rate of female pupae,adult longevity,egg number,egg hatch rate were observed,and the relative expression of EeVg gene were measured by qPCR.[Results]1)The vitellogenin protein gene,namely EeVg(GenBank No.OM804251),was cloned and identified from E.elutella.Its ORF length was 5 337 bp,encoding 1,778 amino acids.2)The relative expression level of the EeVg gene was highest in the fat body of the female adult,which was 17.96 times that of the head.3)X-ray irradiation at 100 and 150 Gy significantly down-regulated the relative expression of EeVg in female pupae after emergence by 0.34 and 0.09 times,respectively.4)X-ray irradiation at doses of 50,100,and 150 Gy reduced the pupation rate and adult lifespan of the tobacco powder moth(87.78％～75.56％and 6.40～4.17 d),significantly reducing the oviposition quantity and egg hatching rate of female moths(55.27～20.37 eggs and 76.67％～72.22％).[Conclusion]X-ray irradiation stress down-regulated EeVg gene expression and significantly reduced the fecundity of female adults,which lays a foundation for further study of the molecular mechanism of X-ray affecting the fecundity of E.elutella.
万方数据
维普
