NtJAZ9基因参与调控烟草腺毛发生的功能研究
Functional Study on the Role of NtJAZ9 Gene in Regulating the Formation of Tobacco Glandular Trichomes
朱文奇 1张新要 2裴文毅 1季盈彤 1董千锋 1廖子鑫 1闫筱筱 1崔红1
作者信息
- 1. 河南农业大学,烟草学院,郑州市郑东新区平安大道218号 450046
- 2. 湖南中烟工业有限责任公司技术中心,湖南省长沙市劳动中路390号 410007
- 折叠
摘要
[目的]探究茉莉酮酸调控烟草腺毛发生的分子机制.[方法]对茉莉酮酸信号关键抑制子NtJAZ9 进行同源克隆、生物信息学分析和基因功能研究.[结果](1)NtJAZ9 有两条同源序列NtJAZ9-1 和NtJAZ9-2,编码区长度分别为 1008 bp和 996 bp.(2)亚细胞定位结果表明NtJAZ9 蛋白定位在细胞核中.在各组织中NtJAZ9-1 相对表达量均高于NtJAZ9-2,NtJAZ9-1 在茎表皮中表达量最高,NtJAZ9-2 在叶中表达量最高.(3)NtJAZ9 过表达植株叶面腺毛密度降低,NtJAZ9 基因敲除植株腺毛密度显著增加,保护毛密度差异不显著.茉莉酮酸甲酯(methyl jasmonate,MeJA)可显著促进长柄腺毛的发生,喷施后过表达株系长柄腺毛密度增加 1.76 倍,敲除株系长柄腺毛增加 0.36 倍.[结论]NtJAZ9基因参与调控烟草腺毛的发生,并在一定程度上影响了MeJA信号对烟草腺毛发生的调控作用.
Abstract
[Purpose]This study aims to explore the molecular mechanism of jasmonic acid in regulating tobacco trichomes glandular trichomes occurrence.[Methods]Homologous cloning,bioinformatics analysis and gene function studies of NtJAZ9,a key repressor of jasmonic acid signaling,were conducted[Results](1)NtJAZ9 has two homologous sequences,NtJAZ9-1 and NtJAZ9-2,with coding regions of 1008 bp and 996 bp,respectively.(2)Subcellular localization results indicate that the NtJAZ9 protein is localized in the nucleus.In various tissues,the relative expression of NtJAZ9-1 is higher than that of NtJAZ9-2,with the highest expression of NtJAZ9-1 in stem epidermis and the highest expression of NtJAZ9-2 in leaves.(3)Overexpression of NtJAZ9 results in reduced glandular trichome density on leaves,while knockout of the NtJAZ9 gene significantly increases glandular trichome density;the difference in protective trichome density is not significant.Methyl jasmonate(MeJA)can significantly promote the occurrence of long-stalk glandular trichomes.After spraying,the density of long-stalk glandular trichomes increased by 1.76 times in overexpressed strains and by 0.36 times in knockout strains.[Conclusion]The NtJAZ9 gene is involved in the regulation of occurrence of tobacco glandular trichomes and affects the regulatory role of MeJA signals on the formation of tobacco glandular trichomes to a certain extent.
关键词
烟草/NtJAZ9/过表达/基因编辑/腺毛密度/MeJAKey words
tobacco/NtJAZ9/overexpression/gene editing/glandular trichome density/MeJA引用本文复制引用
基金项目
国家自然科学基金青年基金(32102361)
湖南中烟工业有限责任公司合作项目(202143000834089)
出版年
2024
国家科技期刊平台
NSTL
万方数据