Isolation and identification of microalgae in tobacco seedbeds in Guizhou region
[Background and Aims]Microalgae are one of the main biological hazards during the tobacco seedling stage,aiming to clarify the algal species in the seedbed.[Methods]The plate streaking method was used to isolate and purify microalgae,and they were identified using a combination of morphological and molecular biology methods.[Results]A total of 67 strains of microalgae were isolated and classified into 7 categories based on morphological characteristics such as color,shape,and size.The cell color of microalgae was various shades of green,with cell shapes including spherical,broad spindle,oval,long cylindrical,and fusiform,and algal cell sizes ranging from 3 to 20 μm.Molecular biological identification showed that the microalgae belonged to 11 genera of the Chlorophyta phylum,with 36 strains of Chlorococcum.[Conclusion]:The dominant algal species in tobacco seedbeds were Chlamydomonas,Chlorella,and Chlorococcum,providing pathogen information for microalgae control.
tobacco seedbedmicroalgaemorphological characteristicsdominant algal species
万方数据
维普
