贵州地区烟草苗床微藻的分离鉴定

Isolation and identification of microalgae in tobacco seedbeds in Guizhou region

周凤 ¹叶果 ¹王清 ²蔡刘体 ³李金梦 ²陈兴江 ³邱雪柏 ³谢伶俐 ⁴汪汉成³

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作者信息

1. 长江大学生命科学学院,荆州 434025;贵州省烟草科学研究院,贵阳 550081
2. 贵州省烟草科学研究院,贵阳 550081;长江大学农学院,荆州 434025
3. 贵州省烟草科学研究院,贵阳 550081
4. 长江大学农学院,荆州 434025
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摘要

[背景和目的]微藻是烟草育苗期的主要生物危害之一,为明确苗床中的藻种.[方法]采用平板划线法分离纯化微藻,运用形态学和分子生物学相结合的方法进行鉴定.[结果]共分离微藻 67 株,根据颜色、形状、大小等形态学特征划分为 7 类.微藻细胞颜色均为不同程度的绿色,细胞形状有球形、宽纺锤形、椭圆形、长圆柱形和梭形,藻细胞大小 3～20 μm.分子生物学鉴定结果表明,微藻均属于绿藻门(Chlorophyta)的 11 个属,其中衣藻属(Chlamydomonas)36株,占 53.73%,小球藻属(Chlorella)10 株,14.92%;绿球藻属(Chlorococcum)6 株,8.96%.[结论]烟草苗床的优势藻种为衣藻属、小球藻属和绿球藻属,结果为微藻防控提供病原信息.

Abstract

[Background and Aims]Microalgae are one of the main biological hazards during the tobacco seedling stage,aiming to clarify the algal species in the seedbed.[Methods]The plate streaking method was used to isolate and purify microalgae,and they were identified using a combination of morphological and molecular biology methods.[Results]A total of 67 strains of microalgae were isolated and classified into 7 categories based on morphological characteristics such as color,shape,and size.The cell color of microalgae was various shades of green,with cell shapes including spherical,broad spindle,oval,long cylindrical,and fusiform,and algal cell sizes ranging from 3 to 20 μm.Molecular biological identification showed that the microalgae belonged to 11 genera of the Chlorophyta phylum,with 36 strains of Chlorococcum.[Conclusion]:The dominant algal species in tobacco seedbeds were Chlamydomonas,Chlorella,and Chlorococcum,providing pathogen information for microalgae control.

关键词

烟草苗床/微藻/形态学特征/优势藻种

Key words

tobacco seedbed/microalgae/morphological characteristics/dominant algal species

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出版年

2024

中国烟草学报

中国烟草学会

中国烟草学报

CSTPCDCSCD北大核心

影响因子：1.182

ISSN：1004-5708

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