Effects of biochar application frequency on the bacterial community in growing soil
[Background]The study aims to assess the impact of biochar application frequency on the bacterial community in continuous tobacco-growing soil.[Methods]Three treatments were set up:a control group without biochar(B0),a treatment with 10 tons of biochar applied annually(B10),and a treatment with 30 tons of biochar applied in the initial year(B30).The effects of biochar application frequency on soil nutrients,enzyme activity,and bacterial communities were analyzed after three years of continuous tobacco planting.[Results](1)Compared to the control(B0)treatment,biochar application significantly increased the total nitrogen and organic carbon content in tobacco-growing soil,with a more pronounced effect in the B10 treatment.Among the treatments,B10 significantly enhanced soil microbial biomass carbon,microbial biomass nitrogen,phosphatase,urease,sucrase,and catalase activities.(2)Compared to B30,the heip and shannoneven indices in the B10 treatment increased significantly by 42.86%and 5.96%,respectively,improving soil bacterial evenness and diversity.(3)Biochar application significantly increased the expression of soil bacterial aromatic compound degradation functions while significantly reducing the relative abundance of tobacco pathogens.[Conclusion]The frequency of biochar application significantly affected the composition of bacterial communities.Annual biochar application in fixed amounts was more effective in improving soil,providing theoretical support for the application of biochar in continuous cropping tobacco growing soil.
万方数据
维普
