[Background and Objective]Arbuscular mycorrhizal fungi(AMF)play a significant role in plant growth promotion and soil improvement.By analyzing the infection characteristics and community structure of AMF in the root systems of flue-cured tobacco(Arbuscular Mycorrhizal Fungi,AMF)in Hunan's tobacco-growing regions,and examining their relationship with environmental factors,this study provides theoretical support for the research and application of mycorrhizal technology.[Methods]Using K326 and Yunyan 87 as experimental materials,comparative trials were conducted in Changsha(CS),Hengyang(HY),Western Hunan(XX),and Yongzhou(YZ).Samples were taken at maturity,and root pressing slides and MiseqPE300 high-throughput sequencing of amplicons were performed.[Results]The degree of AMF infection in K326 and Yunyan 87 showed regional variations,with HY>YZ>XX>CS for K326,and YZ>HY>XX>CS for Yunyan 87.The root AMF diversity index showed regional differences,with XX significantly higher than CS and HY significantly higher than CS.The genus Glomus had the highest relative abundance in all samples.The XX region was highly enriched in Acaulospora,and the CS region was highly enriched in Claroideoglomus,with significant differences between tobacco varieties(P<0.05).Mantel tests indicated that mycorrhizal infection indices of tobacco roots were extremely significantly positively correlated with available nitrogen(AN),total potassium(TK),organic matter(OM),and total phosphorus(TP)(P<0.01).The relative abundance of Acaulospora was extremely significantly positively correlated with elevation(EL)(P<0.01);the relative abundance of Septoglomus was significantly positively correlated with available phosphorus(AP)in K326(P<0.05)and with total potassium(TK)in Yunyan 87(P<0.05).[Conclusion]The diversity,community structure,and infection characteristics of AMF in the root systems of Hunan flue-cured tobacco show regional and varietal specificity.Among the environmental factors,total potassium,alkaline nitrogen,total phosphorus,and organic matter,excluding varietal factors,are the major environmental influences.Future applications of mycorrhizal technology in flue-cured tobacco planting should fully consider the variety and regional environmental conditions.Glomus is the dominant indigenous genus of AMF in Hunan tobacco-growing areas,and its symbiotic effects should be fully utilized.
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