Detection of EGFR,KRAS and PIK3CA gene mutations in esophageal squamous cell carcinoma and analysis of their clinical significance
Objective To analyze the mutations of epidermal growth factor receptor(EGFR),kirsten ratsarcoma viral oncogene(KRAS),phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha(PIK3CA)gene in esophageal squamous cell carcinoma(ESCC)tissue and their relationship with clinical characteristics.Method DNA extraction and target site amplification were performed on tissue samples from 210 esophageal squamous cell carcinoma patients diagnosed at the First Affiliated Hospital of Xinjiang Medical University from January 2006 to December 2012.Then,first generation sequencing was used to sequence EGFR exons 18 and 21,KRAS exon 2,and PIK3CA exon 9.The mutation status of each gene and its relationship with clinical and pathological characteristics were analyzed,and the correlation between different gene mutations was also analyzed.Result The mutation rate of EGFR gene,KRAS gene and PIK3CA gene in 210 ESCC samples was 27.14％,14.29％and 18.59％respectively;The combined mutation rate of EGFR and KRAS was 4.76％,the combined mutation rate of EGFR and PIK3CA was 5.71％,and the combined mutation rate of EGFR,KRAS and PIK3CA was 1.43％;EGFR gene mutation was related to gender,tumor diameter and degree of differentiation(P＜0.05),KRAS gene mutation was related to tumor diameter,degree of differentiation,T stage and clinical stage(P＜0.05),and PIK3CA gene mutation rate was related to gender,tumor diameter and degree of differentiation(P＜0.05);The combined mutation rate of EGFR and PIK3CA genes was related to sex,tumor diameter and differentiation degree(P＜0.05).And there is correlation between EGFR gene and KRAS gene mutation(P＜0.05),while there is no correlation between EGFR gene and PIK3CA gene mutations(P＞0.05).Conclusion The mutation rate of EGFR gene is the highest in ESCC,the mutation rate of PIK3CA gene is the second,and the mutation rate of KRAS gene is the lowest.The combined mutation of EGFR and KRAS gene or EGFR and PIK3CA gene has certain clinical significance.
万方数据
维普
