Correlation analysis between the traditional Chinese medicine certificate type of the old treatment of tuberculosis and cell multiflers and immune expression
Objective The expression level of cytokines and TB lymphocyte subsets in the serum of elderly patients with pulmonary tuberculosis was studied,and the relationship between TCM syndrome and cytokine and TB lymphocyte expression was explored.Method A total of 322 elderly patients with pulmonary tuberculosis who were finally diagnosed with pulmonary tuberculosis from May 2016 to December 2021 due to treatment at the Eighth Medical Center of the Chinese PLA General Hospital were collected,and were divided into four groups according to dialectical classification:lung yin deficiency,yin deficiency and fire prosperity,qi and yin deficiency,and yin and yang deficiency.The expression levels of human interferon-γ(IFN-γ),tumor necrosis factor α(TNF-α),interleukin-β(IL-β),IL-5,IL-2,IL-6,IL-8,IL-17,IL-4and IL-10 were measured in serum by enzyme-linked immunosorbent assay.The absolute number of CD3+,CD4+,CD8+,B cells,NK cells,CD4+/CD8+ in TB lymphocyte subsets were determined by flow cytometry.Result There was no significant difference in the expression levels of IL-β,IL-2,IL-17,IL-5,IL-4 and IL-10 among patients with different TCM syndrome types(P＞0.05).The expression levels of IFN-γ,TNF-α,IL-6 and IL-8 were statistically significant(P＜0.05)in the patients with different TCM syndrome types,and the expression level of IFN-γ in the qi and yin deficiency group was significantly higher than that in the other groups,and there was no significant difference between the two groups(P＞0.05).There was no significant difference in the comparison between the two groups(P＜0.05).The expression level of IL-6 in the yin and yang deficiency group,qi and yin deficiency group,yin deficiency and fire wang group,and lung yin deficiency group decreased in turn,and the difference was statistically significant(P＜0.05).The expression levels of IL-8 was the highest in the yin and yang deficiency group,followed by the qi and yin deficiency group,and the expression level was higher than that in the lung yin deficiency group,yin deficiency and fire wang group,lung yin deficiency group,and There was no significant difference in expression level between patients in the yin deficiency and fire wang group(P＞0.05).In terms of the absolute number of CD3+,CD4+,CD8+,B cells and NK cells,the expression levels of the four groups were significantly lower in the yin and yang deficiency group,qi and yin deficiency group,yin deficiency and fire wang group,and lung yin deficiency group,and the difference between groups was statistically significant(P＜0.05),and the expression level of CD4+/CD8+ was significantly higher than that in the other groups(P＞0.05),and the difference between the other groups was not statistically significant.Conclusion There are differences in the expression of cytokines and immune cells in elderly patients with different syndrome types,which may be used as one of the objective reference standards for dialectical treatment of traditional Chinese medicine,and provide a certain reference for clinical dialectical treatment.
Tuberculosis in the elderlyTraditional Chinese medicine certificate typeCytokinesLymphocyte subsetDialectical governance
万方数据
维普
