子痫前期胎盘组织中神经型Wiskott-Alrdich综合征蛋白的表达及其意义
Expression and significance of N-WASP in placentas with preeclampsia
张硕 1葛云鹏 1王婷婷 1沈鸿飞 1李佳钋 1宋贵玉 1乔宠2
作者信息
- 1. 中国医科大学附属盛京医院妇产科,沈阳 110039
- 2. 中国医科大学附属盛京医院妇产科,沈阳 110039;国家卫生健康委员会生殖健康与遗传医学重点实验室,沈阳 110039;辽宁省母胎医学重点实验室,沈阳 110039;中国医科大学出生队列研究中心,沈阳 110039
- 折叠
摘要
目的 研究子痫前期胎盘组织中神经型Wiskott-Alrdich综合征蛋白(N-WASP)的表达情况及其临床意义.方法 选取2020年7月至2021年12月于中国医科大学附属盛京医院分娩的65例孕产妇作为研究对象,并将其分为早发型子痫前期组(15例)、早发对照组(15例)、晚发型子痫前期组(15例)、晚发对照组(20例).采用实时定量PCR(RT-qPCR)检测各组胎盘组织中N-WASP mRNA的相对表达量,采用Western blotting和免疫组织化学法检测各组胎盘组织中N-WASP 蛋白的表达情况及定位情况.结果 RT-qPCR、Western blotting结果显示,早发型子痫前期组胎盘组织N-WASP mRNA和蛋白表达水平显著低于早发对照组(0.50±0.19 vs.0.93±0.73,0.35±0.17 vs.0.72±0.21),晚发型子痫前期组胎盘组织中N-WASP mRNA和蛋白表达水平显著低于晚发对照组(0.83±0.34 vs.1.15±0.34,0.39±0.16 vs.0.76±0.20),差异均有统计学意义(均P＜0.05).N-WASP mRNA在胎盘组织中的表达与早发型及晚发型子痫前期的发生均呈负相关(r =-0.37,P = 0.042;r =-0.39,P = 0.019).免疫组织化学检测显示N-WASP蛋白定位于合体滋养细胞、细胞滋养细胞、绒毛间质细胞和血管内皮细胞的胞质中.结论 N-WASP低表达可能与子痫前期的发生密切相关.
Abstract
Objective To study the expression and clinical significance of neural Wiskott-Alrdich syndrome protein(N-WASP)in pla-centas with preeclampsia.Methods This study included a total of 65 pregnant women:15 in the early-onset preeclampsia group,15 in the early-onset control group,15 in the late-onset preeclampsia group,and 20 in the late-onset control group.Real-time fluorescence quan-titative PCR(RT-qPCR)was used to detect the relative expression of N-WASP mRNA in placental tissues.Western blotting and immu-nohistochemistry were used to detect the expression and position of N-WASP protein in placental tissues from each group.Results RT-qPCR revealed significantly lower N-WASP mRNA expression levels in the placental tissue of the early-onset preeclampsia group compared to those in the early-onset control group(0.50±0.19 vs.0.93±0.73,P＜0.05).The N-WASP mRNA expression levels in late-onset preeclampsia placenta were significantly lower than those in the late-onset control group(0.83±0.34 vs.1.15±0.34,P＜0.05).Western blotting revealed significantly lower N-WASP protein expression in the placental tissue of early-onset preeclampsia compared to that in the early-onset control group(0.35±0.17 vs.0.72±0.21,P＜0.05).The N-WASP protein expression in late-onset preeclampsia placenta was significantly lower than that in the late-onset control group(0.39±0.16 vs.0.76±0.20,P＜0.05).The N-WASP mRNA expression in the placenta negatively correlated with the occurrence of early-onset(r =-0.37,P = 0.042)and late-onset preeclampsia(r =-0.39,P = 0.019).Immunohistochemistry revealed that N-WASP protein was localized in the cytoplasm of syncytiotrophoblasts,cytotrophoblasts,villous stromal cells,and vascular endothelial cells.Conclusion The low expression of N-WASP may be closely associated with preeclampsia.
关键词
神经型Wiskott-Alrdich综合征蛋白/子痫前期/滋养细胞Key words
neural Wiskott-Alrdich syndrome protein/preeclampsia/trophoblast引用本文复制引用
基金项目
国家自然科学基金(81370735)
国家自然科学基金(81771610)
百千万人才工程领军人才项目(XLYC2005008)
辽宁省民生科技计划联合计划(2021JH2/10300123)
沈阳市科技计划(20-205-4-004)
出版年
2024
国家科技期刊平台
NSTL
维普
万方数据