Expression and significance of nuclear factor-κB and interleukin-6 in radiation-induced bladder injury
Objective To detect the expression of nuclear factor-κB(NF-κB)and interleukin-6(IL-6)in radiation-induced bladder injury and analyze their relationship.Methods SV-HUC-1 cells were cultured and irradiated with X-ray doses of 5,10,and 15 Gy for 24,48,and 72 h,respectively,and those not irradiated were used as a normal control group.Proliferative activity of SV-HUC-1 cells was detected using a CCK-8 assay,and the expression of NF-κBand IL-6mRNA was detected by real-time quantitative PCR.An siR-NA-NF-κB plasmid was transfected into SV-HUC-1 cells following irradiation with 15 Gy for 72 h to establish the si-NF-κB group,and cells not transfected following irradiation with 15 Gy for 72 h was used as a blank control group.Expression of IL-6 protein was detected by Western blotting.Twenty male SD rats were divided into a model group(receiving 20 Gy irradiation to establish a model of radia-tion-induced bladder injury)and control group(n= 10 per group).The expression of NF-κBand IL-6mRNA in the rat urinary bladder tissues was detected by real-time quantitative PCR.Results With an increase in irradiation dose and time,the proliferative activity of SV-HUC-1 cells decreased,and the expression of NF-κBand IL-6mRNA increased.The expression of IL-6 was significantly lower in the si-NF-κB group than in blank control group.The expression of NF-κBand IL-6mRNA in the rat urinary bladder tissues was higher in the model group than in control group,and a positive correlation was observed between the expression of NF-κBand IL-6 mRNA.Conclusion Damage caused by X-rays to the urinary bladder is concentration-and time-dependent.High expression and syner-gistic effect of NF-κB and IL-6 lead to the formation of radiation-induced bladder injury.
国家科技期刊平台
NSTL
万方数据
维普
