长链非编码RNA FGD5-AS1调节miR-195-5p/PIM1轴对高糖诱导的心肌细胞损伤的影响
Effect of long non-coding RNA FGD5-AS1 on high glucose-induced myocardial cell injury through regulation of the miR-195-5p/PIM1 axis
谭冰 1方凌燕 2陈明华 3曾巧莉 4郭润民4
作者信息
- 1. 广东医科大学 附属第二医院内分泌科,广东 湛江 524000
- 2. 广东医科大学附属第二医院心血管科,广东 湛江 524000
- 3. 广东医科大学附属第二医院神经内科,广东 湛江 524000
- 4. 广东医科大学顺德妇女儿童医院,佛山市顺德区妇幼保健院科教科,广东 佛山 528300
- 折叠
摘要
目的 探讨长链非编码RNA FGD5反义RNA1(lncRNA FGD5-AS1)调节miR-195-5p/PIM1轴对高糖诱导的心肌细胞损伤的影响.方法 将大鼠心肌细胞H9c2及人心肌细胞随机分为对照组、模型组、lncRNA FGD5-AS1过表达组、miR-195-5p inhibitor组、阴性对照组、lncRNA FGD5-AS1过表达+miR-195-5p mimics组,分组处理后检测其lncRNA FGD5-AS1、miR-195-5p和PIM1表达、增殖、凋亡、促炎性细胞因子水平.结果 与对照组相比,模型组细胞lncRNA FGD5-AS1、PIM1mRNA及蛋白表达、存活率,增殖率降低(P<0.05);miR-195-5p表达,凋亡率,肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)水平升高(P<0.05);过表达lncRNA FGD5-AS1可逆转模型组细胞上述变化,上调miR-195-5p可减弱过表达lncRNA FGD5-AS1的逆转作用.结论 过表达lncRNA FGD5-AS1可通过下调miR-195-5p增强PIM1表达,从而抑制高糖诱导的心肌细胞炎症反应,促进细胞存活,减轻细胞凋亡.
Abstract
Objective To investigate the effect of the long non-coding RNA FGD5 antisense RNA1(lncRNA FGD5-AS1)on myocar-dial cell injury induced by high glucose levels through regulation of the miR-195-5p/Pim-1 proto-oncogene(PIM1)axis.Methods The rat myocardial cell line H9c2 and human myocardial cells were randomly allocated to the control,model,lncRNA FGD5-AS1 overex-pression,miR-195-5p inhibitor,negative control,and lncRNA FGD5-AS1 overexpression+miR-195-5p mimic groups to determine the expression of lncRNA FGD5-AS1,miR-195-5p,and PIM1.In addition,cell proliferation,apoptosis,and pro-inflammatory cytokine levels of the cells in each group were analyzed.Results Compared with the control group,the protein and mRNA expressions of lncRNA FGD5-AS1 and PIM1 and the survival and proliferation rates in H9c2 and human myocardial cells in the model group decreased(P<0.05),and the expression of miR-195-5p,apoptosis rate,and production levels of tumor necrosis factorα(TNF-α)and interleukin-6(IL-6)increased(P<0.05).Overexpression of the lncRNA FGD5-AS1 reversed these pathological changes in model group cells,whereas upregulation of miR-195-5p could weaken the effects of overexpression of lncRNA FGD5-AS1.Conclusion Overexpression of lncRNA FGD5-AS1 enhances the expression of PIM1 by downregulating miR-195-5p,thereby inhibiting high glucose-induced myocyte inflamma-tion,promoting survival and growth,and alleviating apoptotic injury.
关键词
长链非编码RNA/FGD5-AS1/miR-195-5p/PIM1/高糖/心肌细胞/损伤Key words
lncRNA FGD5-AS1/miR-195-5p/PIM1/high glucose/myocardial cell/injury引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据