Study of resveratrol-mediated SIRT2 intervention in dexamethasone-induced mitophagy in bone marrow mesenchymal stem cells
Objective To investigate the mechanism of resveratrol(Res)-intervening dexamethasone(Dex)-induced mitochondrial autophagy in bone marrow mesenchymal stem cells(BMSCs)through SIRT2.Methods Mouse osteoblastic BMSCs were treated with Res,Dex,and the SIRT2 antagonist NAM.The mitochondria autophagosomes were observed using transmission electron microscopy(TEM).The protein and mRNA expression levels of SIRT2,LC-3,Beclin-1,TOM20,and Hsp60were determined using Western blot-ting and reverse transcription-polymerase chain reaction(RT-PCR).Results Res enhanced SIRT2 expression in BMSCs in a dose-and time-dependent manner.Dex(10-6 mol/L)inhibited the proliferation and viability of BMSCs,and significantly down-regulated the expres-sion of SIRT2mRNA and protein in BMSCs,whereas Res(10-6 mol/L)significantly inhibited the negative regulatory effects of Dex on the proliferation of and SIRT2 expression in BMSCs.Res(10-6 mol/L)significantly increased the mRNA expression of LC-3and Beclin-1(P<0.05),but significantly decreased the protein expression of TOM20 and Hsp60(P<0.05).Conclusion Res plays a role in the regulation of Dex-induced mitochondrial autophagy in BMSCs by mediating SIRT2.
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维普
