Application of Real-Time PCR in Nucleic Acid Detection in Blood Stations
Objective:To observe and analyze the application effect of real-time quantitative PCR in nucleic acid detection in blood stations.Methods:Samsamples of 17564 from January 2023 to June 2023 were selected for HBsAg,anti-HCV,anti-HCV,anti-HIV,anti-TP,ALT and reexamination for nucleic acid detection by real-time PCR.Results:In the blood samples of 17564 voluntary blood donors,a total of 25 cases were detected as positive for hepatitis B virus deoxyribonucleic acid,and 0 cases as positive for hepatitis C virus ribonucleic acid and human immunodeficiency virus(type 1)ribonucleic acid using real-time fluorescence quantitative PCR technology.In the inter room quality assessment conducted at the clinical inspection center,all inspection conclusions were correct,with a score of 100 points.Conclusion:Real-time quantitative PCR technology used in nucleic acid detection in blood stations can effectively reduce the virus detection window period and contribute to the improvement of blood safety level.
万方数据
维普
