Application of Automatic Blood Group Analyzer and Microplate Method in ABO Blood Group Screening
Objective:To explore the application value of automatic blood group analyzer and microplate method in ABO blood group screening.Methods:The blood donors collected from January 2022 to April 2023 in our hospital were studied.80 blood donors were all used as research subjects.80 anticoagulant samples of EDTA were collected from all subjects and tested by microplate method and automatic blood group analyzer respectively.The results of blood group screening,positive and negative stereotyping of ABO blood group and irregular antibody of O cell condensation were compared,and the clinical application value of different detection methods was analyzed.Results:Automatic blood group analyzer for the sample subtype missed rate was low,O cell aggregation rate was high,but the two methods had no significant difference in the detection rate(P>0.05).The ABO subtype,positive and negative type inconsistency rate and antibody reduction rate of automatic blood group analyzer were slightly higher,and the normal blood group rate was low,but there was no significant difference between groups(P>0.05).The detection rate of O cell agglutination irregular antibody by automatic blood group analyzer was significantly higher than that by microplate method(P<0.05).Conclusion:Both automatic blood group analyzer and microplate method can be used in ABO blood group screening,but the former is more effective and safe,can better detect irregular antibodies,and has a broader application prospect.
automatic blood group analyzermicroplate methodABO blood group screeningirregular antibody