Objective:To prepare a gelatin sponge microsphere for vascular embolization and characterize its physicochemical properties.Its safety and efficacy for embolization was preliminarily verified through in vivo test.Methods:In the preparation of gelatin sponge microspheres,aqueous phase containing gelatin was added into the oil phase containing emulsifying agent,resulting in an oil-in-water emulsion system.Then by adding crosslinking agent,the emulsion system were solidified and transformed into gelatin sponge microspheres.The physical and chemical properties of the prepared microspheres were characterized,including microscopic appearance,particle size,swelling rate and elasticity.The prepared gelatin sponge microspheres were injected into pig renal vessels through microcatheter,and the embolization efficacy and postoperative complications were observed to verify the safety and effectiveness of microspheres for in vivo embolization.Results:The gelatin sponge microspheres obtained by this method were with smooth surface and spherical in shape.After swelling,the particle size conformed normal distribution,the particle size range was basically between 50-2000μm,and the average swelling rate was 355%.Being compressed to around 50%of the diameter through texture analyzer,the microsphere did not break or deform plasticly.In vivo test of pig kidney embolization showed that the microspheres could effectively embolize the target vessels and cause ischemic infarction of the target tissues.Microspheres completely degraded in vivo in 28 days,and the blood vessels were recanalized then.No complications related to gelatin sponge microspheres were observed during the experiment.Conclusion:The gelatin sponge microspheres prepared by this method are spherical with regular shape and wide particle size distribution,and have the potential to be used for vascular embolization for different levels.The microspheres have moderate swelling rate and good elasticity,which are easy to be delivered through the catheter during the operation and embolized the target blood vessels solidly.The results of animal experiments on renal embolization in pigs also showed that the prepared gelatin sponge microspheres had good safety and efficacy,moderate degradation time,indicating great market prospects for vascular embolization.
gelatin spongemicrospheresvascular embolizationpig kidneyin vivo Test
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