Comparative Analysis of HCV Detection in Blood Samples by Nucleic Acid Testing and ELISA Testing
Objective:To analyze the application of nucleic acid detection and enzyme-linked immunodetection(ELISA)for HCV detection in blood samples.Methods:1200 blood samples were tested using nucleic acid testing and ELISA,and the positive rates of the two methods were compared,as well as their diagnostic value for hepatitis C virus infection.Results:Using the HCV RNA detection results as the gold standard,the positive rate of hepatitis C virus detection was 4.00%,with a combined detection positive rate of 3.92%,both higher than the 2.67%of nucleic acid detection and 2.50%of ELISA,with statistically significant differences(P<0.05).The detection value of joint testing is higher than that of nucleic acid testing and ELISA testing(P<0.05).Conclusion:Both nucleic acid testing and ELISA testing have certain value in detecting hepatitis C virus in blood samples.The positive rate of nucleic acid testing is slightly higher than that of ELISA testing.However,in order to improve the disease screening rate,it is necessary to combine the above methods for testing.
nucleic acid detection methodenzyme-linked immunoassayblood sampleshepatitis C virus
万方数据
维普
