The PKC/NF-kB Signaling Pathway was Detected by Electrophoresis to Explore the Anti-Osteoporosis Mechanism of Bionic Artificial Tiger Bone in Ovariectomized Mice
Objective:To explore the anti-osteoporosis mechanism of bionic artificial tiger bone in ovariectomized mice by detecting PKC/NF-kB signaling pathway changes using electrophoresis.Methods:30 SD female mice were randomly divided into control group,operation group and operation+artificial tiger bone group.The control group received sham operation without ovariectomy,and the operation group received ovariectomy.The operation+artificial tiger bone group was given artificial tiger bone meal on the basis of operation group.The serum levels of ALP,BGP,β-CTX,TNF-α,alkaline phosphatase and tartrate-resistant acid phosphatase in each group were observed,the biomechanical properties of femur were measured,and the protein expressions of ALP,BGP,PKC and NF-kB in bone tissue were detected by electrophoresis.Results:The levels of ALP,BGP,β-CTX and TNF-α were significantly different among all groups(P<0.05).Surgical group ALP,β-CTX,TNF-α the level was higher than that of the control group and the surgery+artificial tiger bone group(P<0.05);The BGP level in the surgical group was lower than that in the control group and the surgical+artificial tiger bone group(P<0.05);Alkaline phosphatase and tartrate-resistant acid phosphatase in operation group were higher than those in control group and operation+artificial tiger bone group(P<0.05).There was no significant difference in alkaline phosphatase and tartrate-resistant acid phosphatase between operation+artificial tiger bone group and control group(P>0.05).There were significant differences in the maximum stress,maximum load,fracture load and elastic modulus among all groups(P<0.05).The maximum stress,maximum load,fracture load and elastic modulus of operation group were lower than those of control group and operation+artificial tiger bone group(P<0.05).The relative expressions of BGP in the operation group were lower than those in the control group and the operation+artificial tiger bone group,while the relative expressions of ALP,PKC and NF-kB were higher than those in the control group and the operation+artificial tiger bone group(P<0.05).There was no significant difference between control group and operation+artificial tiger bone group(P>0.05).Conclusion:Biomimetic artificial tiger bone can improve bone metabolism indexes and biomechanical properties of femur in ovariectoid mice to achieve anti-osteoporosis effect,and its mechanism may be related to PKC/NF-kB signaling pathway.
万方数据
维普
