Software Design of Micropressure Control System for Patch Clamp Giga-Ohm Seal
In the field of biomedical research,the patch-clamp technique is a vital electrophysiological tool widely used in neuroscience,cardiovascular science,and pharmacology.In patch-clamp experiments,the formation of a Giga-Ohm seal is a critical step that relies on precise control of gas pressure.However,most current experiments still employ manual pipetting,which suffers from quantitative inaccuracies and cannot provide consistent negative pressure,thus affecting seal quality,experimental efficiency,and high-throughput cell testing.To address these issues,this study designs and implements a micropressure control system that significantly improves the Giga-Ohm seal formation process.This system provides a robust reference for accurate control of gas pressure during patch-clamp experiments,enhancing their accuracy and reproducibility.This advancement not only promotes the broader application of patch-clamp technology in biomedical research but also offers new perspectives and methods for related fields.
patch clampGiga-Ohm sealmicropressure control systemsoftware design