Regulation of Anxiety-Like Behavior in Mice by Electrical Stimulation of the Cortex with a Modified Rehabilitation Apparatus
Objective:To investigate the intervention mechanism of improved rehabilitation equipment electrical stimulation of cortical on anxiety like behaviour deficit induced by partial injury of amygdala in mice.Methods:Transform the probe of the EEG biomimetic electrical stimulator treatment device to make it suitable for C57 mice.C57 mice were selected as subjects.First,performed open field test(OFT)and the elevated plus maze(EPM)on mice;And measured the heart rate of mice under light anesthesia(HR test).Second,injured the bilateral BLA(AP=2.24,ML=±2.90,DV=4.75)of mice,and repeated OFT,EPM and HR test.Then electrical stimulated the mice cortexes,and repeated OFT,EPM and HR test.Results:Partial lesion of bilateral amygdala in mice resulted in significant decrease of anxiety like behavior ability(P<0.05,one way ANOVA),accompanied by a decrease in heart rate(P<0.05,one way ANOVA);After stimulated on cortexes of mice,the anxiety like behaviors ability of mice were increased(P<0.05,one way ANOVA),accompanied by an increase in heart rate(P<0.05,one way ANOVA).Conclusion:BLA injury caused the decrease of anxiety like behavior levels in mice,accompanied with decrease in HR.Electrical stimulation of the cortex can improve the levels of anxiety like behavior in mice,accompanied with increase in HR.
improve equipment probeselectrical stimulationcortexamygdalaanxiety like behaviorheart rate