Application of Next-Generation Sequencing Technology in Microbial Identification
Objective:The method of microbial identification was established by next-generation gene sequencing technology.Methods:For bacterial,V1-V3 region of 16S rRNA gene was amplified.For fungal,ITS1 and ITS2 gene regions were amplified.After labeling,the amplified products were sequenced by iSeq100,and the data were analyzed by the microbial identification system to obtain the identification results.Results:22 strains environmental microorganisms and 31 strains standard microorganisms were identified.At the genus level,the concordance of identification results was 100%.At the species level,the concordance of identification results was 81.1%.Conclusion:The identification methods of bacteria,yeast and mold were successfully established by next-generation sequencing technology.The methods have high success rate,accuracy and reproducibility,can be used to identify environmental microorganisms.
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