Optimization and Practice of Experimental Teaching Methods for Spinal Cord Slices in Rats
Spinal cord slices are commonly used live tissue in electrophysiological experimental for medical graduate postgraduates.The traditional spinal cord slicing method has been employedfor numerous years.Owing to the limitations of experimental technical conditions,this method is merely applicable to young rats.A multitude of optimizations have been carried out on the experimental method in the article,including the addition of heart perfusion surgery,the simplification of the steps of taking the spinal cord and stripping the membrane,as well as the alteration of the solution formula and other measures.The improvedexperimental method has enhanced the activity of neurons to a certain extent,and successfully carried out patch clamp recordings on the spinal cord slices of adult rats.
optimization of experimental methodsspinal cord slicespatch clamp recording