Study on the Source and Detection Method of Disposable Infusion Set Cytotoxicity
Objective:To explore the source of disposable infusion set cytotoxicity and verify a new detection method,in order to provide a guidance for scientific evaluation of disposable infusion set cytotoxicity.Methods:CCK-8 method was used to study the components,catheter,and the raw materials of the infusion set.The conventional weight extraction method and the new perfusion method were combined to evaluate the source of the disposable infusion set cytotoxicity.Results:The CCK-8 method met the quality control requirements in all the experiments in this study.Four kinds of catheter materials[PVC(plasticizer DEHP),PVC(plasticizer TOTM),TPU,TPE]infusion samples were extracted by gram weight method.Two kinds of PVC catheter materials infusion sets showed potential cytotoxicity.As the weight ratio of the PVC catheter increases in infusion set,the overall infusion set cytotoxicity elevates with the use of weight extraction method.More obvious cytotoxicity was observed when the PVC and TPU catheter components of the infusion set were extracted alone using the gram weight method.While no potential cytotoxicity was observed for other components.The raw material of PVC tubing for infusion sets shows potential cytotoxicity.The perfusion method further suggests that infusion sets with PVC and TPU catheter materials are potentially cytotoxic,while TPE catheter materials show no potentially cytotoxic.Conclusion:Disposable infusion set cytotoxicity comes from the infusion set catheter.TPE catheter shows relatively lower cytotoxicity than PVC and TPU.Compared with conventional weight extraction method,the perfusion method is more suitable for the evaluation of disposable infusion set cytotoxicity.
disposable infusion setsources of cytotoxicitydetection methodology