The Influence of Dilution Factor on Bacterial Endotoxin Test
Gradient dilution is often used in bacterial endotoxin testing,and various sources often mention `no more than 10 times dilution',believing that errors will be greater if the dilution exceeds 10 times.The article observes the differences in test results at different dilution ratios through bacterial endotoxin testing,and believes that excluding uncontrollable factors such as individual differences in reagents and consumables,personnel operation errors,etc.,dilution less than 10 times is generally better than dilution greater than 10 times,with 2-fold dilution being the best.Without considering cost,it is recommended to use a 2-fold dilution.