Sensitivity and Specificity Analysis of Bacterial Culture Method and Real-Time Fluorescence PCR for Detecting Colonized Group B Streptococci in Late Pregnancy Pregnant Women
Objective:To analyze the sensitivity and specificity of bacterial culture method and real-time fluorescence PCR in the detection of group B streptococcus (GBS) in late pregnancy pregnant women. Methods:120 late pregnancy pregnant women admitted from June 2021 to July 2023 were selected as the analysis objects,and their reproductive tract rectal secretion swabs were taken as specimens,a total of 2. During the test,bacterial culture method and real-time fluorescence PCR detection were used,and gene sequencing results were used as the gold standard. The diagnostic efficacy of the two detection methods was compared. Results:The genetic sequencing test showed that 52 out of 120 pregnant women were positive and 68 were negative;The bacterial culture results showed 45 positive cases and 60 negative cases;The real-time fluorescence PCR results showed 51 positive cases and 66 negative cases;The sensitivity,specificity,and accuracy of implementing fluorescence culture PCR were better than those of bacterial culture (P<0.05). Conclusion:When detecting GBS in late pregnancy pregnant women,the implementation of fluorescence PCR detection can be adopted. This method has a shorter time consumption,high sensitivity and specificity,which helps to clarify the actual situation of pregnant women and provide scientific basis for subsequent treatment.
late pregnancycolonization of group B streptococcusbacterial culture methodreal time fluorescent PCRsensitivityspecificity