The Optimization of High-Performance Liquid Chromatography for Blood Concentration Detection of Vancomycin
Objective:To optimize the application of high-performance liquid chromatography in the detection of vancomycin blood drug concentration. Methods:Plasma samples containing vancomycin were placed in a 10％ perchloric acid methanol acetonitrile mixed solution at a dose of 100 μL for precipitation,with a ratio of 2∶1∶1;Then centrifuge it and select a supernatant with a dose of 150 μL. The above liquid needs to be placed in an inner liner,and the mobile phase needs to be intervened with acetonitrile water,while maintaining a flow rate of 0.8mL/min. The chromatographic column was Agilent ZORBAX SB-C18,and the column temperature is maintained at 40℃. The detection wavelength was 220nm,and it needs to be analyzed. Analyze the various aspects of this method. Results:The linear relationship was good when the blood concentration of vancomycin was maintained within the range of 1.18-75.80 μg/mL,R2=0.9994;The intra-and inter day precision values were all below 15％. Stability studies of vancomycin at low,medium,and high concentrations at room temperature,4℃,and-20℃ showed that its relative standard deviation (RSD) was below 15％,indicating good stability under different temperature conditions. Conclusion:Optimizing high-performance liquid chromatography can effectively determine the blood concentration of vancomycin and is worthy of clinical application.
high-performance liquid chromatographyvancomycinblood drug concentration detectionresearch
万方数据
维普
