Study on the Determination of Styrene Metabolites Concentration in Urine Using Flame Atomic Absorption Spectrophotometer-AA-7020
Objective:To develop a new method for determining the concentration of styrene metabolites in urine using flame atomic absorption spectrophotometer AA-7020. Methods:The urine sample was first acidified with 2％ nitric acid,and then extracted with 20 mL of ether. The extract was dried and dissolved in 5 mL of deionized water before being directly injected into the flame atomic absorption spectrophotometer AA-7020 for detection. Quantitative analysis of the concentration of styrene metabolites in urine was conducted under the conditions of instrument parameters set to wavelength 283.3 nm (a common detection wavelength for styrene metabolites such as hippuric acid,adjusted according to actual conditions),slit width of 0.4 nm,gas flow rate of 2.0 L/min,and co gas flow rate of 1.5 L/min. The key performance indicators of this method,including pre-treatment efficiency,spiked recovery rate (expected range of 90％ to 110％),precision (expected relative standard deviation below 5％),and detection limit (expected below 0.05 μg/mL),were evaluated and compared with the standard method. Results:In the study,the concentration of styrene metabolites in urine showed a good linear relationship with absorbance in the range of 0.1-1.0 μg/mL (correlation coefficient not less than 0.99),with a detection limit of 0.03 μg/mL. The recovery rate remained stable between 95％ and 105％,and the relative standard deviation was less than 3％. Compared with the comparison method,there was no statistically significant difference in the results obtained by the two methods (P>0.05). Conclusion:The method established in this study demonstrates advantages such as simple operation,high sensitivity,good reproducibility,and wide applicability in determining the concentration of styrene metabolites in urine. It provides a reliable and efficient detection method for assessing styrene exposure in urine and has high practical application value.
flame atomic absorption spectrophotometerstyrene metabolites in urineconcentration determination
万方数据
维普
