Effect of extracorporeal shock wave on endothelin-1 expression and content in blood of glucocorticoid-induced femoral head necrosis in rabbits
Objective To investigate the effect of extracorporeal shock wave on the change of endothelin-1 in blood plasma and endothelin-1 expression in glucocorticoid-induced femoral head necrosis rabbits. Methods Fifty-four healthy adult male Japanese rabbits, random selection 18 as control group(group A), other 36 rabbits randomly divided into model group(group B), shock wave therapy group(group C) after established glucocorticoid-induced necrosis models of femoral head, each group have 18 rabbits. Group C were start given shock wave therapy to the femoral head after the dose to the rabbit in the second week, group A group B both didn't give. Each group were randomly selected 6 rabbits take ear arterial blood after the dose to the rabbit in the 4, 6, 8weeks, at the same time use the air embolism method put rabbits to death and get femoral head. Hematoxylin-eosin staining was used to observe the histological changes of glucocorticoid-induced femoral head, enzyme-linked immuno sorbent assay was used to measure osteoprotegerin content in blood of rabbits, immunohistochemical stains observed ET-1 positive expression in glucocorticoid-induced femoral head and the expression was semi-quantitative analysised. Results Plasma ET-1 content:there was no difference between B group and C group at week 4 after the dose to the rabbit(P >0.05), but both was higher than A group(P <0.05). At week 6, 8, there was no difference between C group and A group(P >0.05), but both of them were lower than B group(P <0.05). Positive expression of ET-1 in femoral head: At week 4, 6 and 8 after the dose to the rabbit, no difference between C group and A group was observed(P >0.05), but both of them were lower expression than B group(P <0.05). Conclusion ESW can reduceET-1 expression in femoral head of glucocorticoid-induced femoral head necrosis rabbits and can Induction and strengthen osteeblast proliferation and promote new bone formation in the avascular necrosis of rabbit femoral head.
NETL
NSTL
万方数据
维普
