Objective To explore cavity ultrasound hydrogen peroxide enhance imaging technology in the diagnosis of anal fistula application value. Method Retrospective analysis of preoperative line rectal cavity ultrasound hydrogen peroxide enhanced imaging technical inspection of anal fistula operation, a total of 59 cases of patients, patients will be ultrasound examination results and intraoperative see results are compared and analyzed. Result Rectal intracavitary ultrasonic hydrogen peroxide enhanced imaging for anal fistula main branch judgment accuracy rate is 100%, for anal fistula branch judgment accuracy rate is 87.10%, for anal fistula in mouth judgment accuracy rate is 94.92%. Conclusion Rectal intracavitary ultrasonic hydrogen peroxide enhance imaging technology in the diagnosis of anal fistula of anal fistula main branch location, branch and inner port number and position judgment has high clinical value.