探讨游离脂肪酸对近曲肾小管上皮细胞HK-2的影响及作用机制
Explore free fatty acids to proximal convoluted renal tubular epithelial cells of HK-2 influence and function mechanism
韩乐 1刘晓玲 1荣青峰 1刘琳1
作者信息
- 1. 030001 太原，山西医科大学第二临床医学院内分泌内科
- 折叠
摘要
目的探讨游离脂肪酸(Free Fatty Acids,FFAs)对人近曲肾小管上皮细胞(HK-2)的影响及作用机制.方法用含有不同浓度(0、125、250、500μmol/L)软脂酸的DMEM-F12培养人近曲肾小管上皮细胞(HK-2),在0h、24h、48h三个时段：MTT法检测不同环境下FFAs对HK-2细胞增殖的影响,流式细胞术检测FFAs对细胞凋亡和细胞周期的影响,免疫细胞化学法检测处理前后HK-2细胞中白细胞介素-β(IL-β)、白细胞介素-8(IL-8)表达的变化.结果 FFAs呈剂量和时间依赖性抑制HK-2细胞的增殖(P<0.05),并可能通过阻滞细胞周期,诱导细胞凋亡(P<0.05).经FFAs作用后,IL-β、IL-8的表达增加.结论游离脂肪酸有抑制HK-2细胞增殖,促进其凋亡的作用,同时炎性细胞因子IL-β、IL-8表达增多,参与了其发生和发展的过程.
Abstract
Objective Explore free fatty acid(FFAs) to proximal convoluted tubules epithelial cells(HK-2) influence and function mechanism. Methods People proximal convoluted tubules epithelial cells(HK-2), were cultured with DMEM-F12 of different concentrations of palmitic acid(0, 125, 250. 500μmol) (using defat bovine serum albumin as vector), normal medium as control grop. on the 0 hour, 24 hour, 48 hour respectively: Applyied four methyl azo thiazole blue(determined by MTT) colorimetric detection different environment HK-2 cell proliferation capacity;Streaming cells in cell apoptosis FFAs intraoperative detection and the influence of the cell cycle; Immune cell chemical method before and after treatment were detected in HK-2 cells interleukin-β(IL-β), interleukin-8 (IL-8) expression changes. Results In FFAs doses and time dependence inhibit HK-2 cell proliferation(P<0.05), and possibly by blocking the cell cycle and apoptosis(P<0.05). After FFAs role by IL-β, IL-8 expression increase. Conclusion Free fatty acid HK-2 it inhibits cell proliferation, promote its apoptosis role, and inflammatory cells factor IL-β, IL-8 express increasing, participated in its occurrence and development process.
关键词
游离脂肪酸/肾小管上皮细胞/白细胞介素-1β/白细胞介素-8/凋亡/增殖Key words
Free fatty acids/Renal tubular epithelial cells/Interleukin-1β/Interleukin-8/Apoptosis/Proliferation引用本文复制引用
基金项目
出版年
2013
NETL
NSTL
维普
万方数据