Cellular immunity research on combination transplantation of antogeneic Splenic cell and slices after rat's splenectomy
Objective To observe functional restoration of pristine cellular immunity after splenic sliices autotransplantation by establishing animal model on combination trantran-splantation of antogeneic Splenic cell and slices. Methods 48 wistar rats are randomly divid ed into four groups, A group: splenectomy. B group: splenic slices transplantation. C group: spleen cell transplantation. D group: combination transplantation. Before animal moddel and within 1,4,8 weeks after stablishing animal model, peripheral blood is drewn and detected the level of peripheral blood T-cyte subset CD3+,CD4+,CD8+ and CD4+/CD8+, At the same time, the results are compared. Result In A group postoperative CD3+,CD4+,CD8+ became lower(P<0.05); In B group CD3+,CD4+,CD8+ became lower compared with preoperative levels at the postoperative 1th and 4th week(P<0.05), got close common at the postoperative 8th week(P>0.05); In C group CD3+,CD4+,CD8+ became lower compared with preoperative levels at the postoperative 1th week(P<0.05), got close common at the postoperative 4th week(P>0.05), but became gradually lower at the postoperative 8th week(P<0.05); In D group CD3+,CD4+,CD8+ became lower compared with preoperative levels at the postoperative 1th week(P<0.05), got close common at the postoperative 4th and 8th week(P>0.05); Statistically difference was detected between the C, D and A, B groups for CD3+,CD4+,CD8+ at the postoperative 1th and 4th week(P<0.05), Statistically difference was detected between the B, D and A, C groups for CD3+,CD4+,CD8+ at the postoperative 8th week(P<0.05). Conclusion Combination trantransplantation of antogeneic Splenic cell and slices can retrieve partial pristinel cellular immune function.
NETL
NSTL
万方数据
维普
