Effect of the δ-opioid Receptor Agonist DADLE on lung water transport after Global Cerebral Ischemia and Reperfusion in Rats
Objective To occlusion combined with hypotension to establish the rat model of cerebral ischemia reperfusion by two vessels, observation of δ?opioid receptor agonist DADLE on the transport of water in the lung in rats, to explore its on cerebral ischemia reperfusion injury(ALI) and the protective effect of. Methods 30 healthy SD rats were randomly divided into sham operation group(Sham), model group(I/R), DADLE treatment group. Sham operation group(Sham, n=10): expose only blood vessels and no closed; ischemia reperfusion group(I/R, n=10): using improved two vessel occlusion of cerebral ischemia reperfusion model combined with hypotension, 15min after ischemia reperfusion was given 120min; DADLE treatment group(n=10) on reperfusion first through the left jugular venous injection of DADLE5mg/kg, 120min after reperfusion, and the right lung tissue, morphological changes were observed by routine HE staining, left lung tissue wet weight/dry weight(W/D) value. Compared with the sham operation group(Sham), model group(I/R) were analyzed. Results After injection of the lung showed alveolar septum, capillary dilatation and congestion, alveolar cavity and perivascular infiltration of neutrophils, tracheal wall portion of epithelial cell, lung and tracheal cavity are serous fluid exudation. DADLE treatment group reduced lung congestion, neutrophil infiltration decreased. Conclusion Acute cerebral ischemia reperfusion rat models with different degrees of damage to the lungs, DADLE can reduce lung fluid excess secretion, has a protective effect on ALI.
DADLECerebral ischemia reperfusionLung injuryLung water transport
NETL
NSTL
万方数据
维普
