Breeding reproducing and genotype identifying IL-2 knockout mice
Objective To breed, reproduce and identify IL-2 knockout mice. Methods Heterozygote mice were bred and reproduced. Genome DNA extracted from the murine tail was subjected to PCR fog genotype identification. Result The genotypes of the offspring mice were heterozygous(IL-2+/-), homozygous(IL-2-/-), and wild-type(IL-2+/+). Conclusion Breeding and reproducing of IL-2 knockout mice were successful, which provide a necessary conditions for the further study of IL-2 deletion affect immune function.
NETL
NSTL
万方数据
维普
