Effects of DADLE on Bcl-2 and Caspase-3 of rats’ brain expression following cerebral- ischemia reperfusion injury
Objective To determine expression of Bcl-2 and Caspase-3 in brain tissue of rat before and after using DADLE, and to investigate effects of DADLE on Bcl-2 and Caspase-3 expression following global cerebral- ischemia reperfusion injury and the relationship with Brain protection. Methods 50 Sprague-Daw-ley(SD) rats were randomly entolled into 5 groups: the sham-operated: only exposure blood vessels; the ischemia reperfusion group(I/R, n=10):improved establishment of two vessel occlusion add hypotensionand establish global cerebral-ischemia reperfusion, 15min after ischemia and give reperfusion; three DADLE-treated(different drug doses: 2mg/kg[A], 3mg/kg[B] and 5mg/kg[C]):via external jugular vein injection of DADLE before reperfusion on the basis of the I/R group. Each animal Put to death after reperfusion for 120min, Craniotomy for brain tissue, Using western-bolt technique to detect the expression of Caspase-3 and Bcl-2 in brain tissue of rat. Results Western-bolt test results: The expression level of Bcl-2 in sham group was significantly lower than that of other groups(P<0.05), and only a small amount of Caspase-3 expression in Sham group. DADLE group compared with I/R group, Bcl-2 expression level increased(P<0.05) and Caspase-3 expression level decreased(P<0.05). Each group of Bcl-2 between B group and C group expression was higher than that in A group(P<0.05), B group and C group had no difference(P>0.05); the expression level of Caspase-3 in B group and C group was significantly lower than that in group A expression(P<0.05), B group and C group differences no statistical significance(P>0.05). Conclusion Substance DADLE may through the up-regulation of Bcl-2 expression and inhibit the expression of Caspase-3, inhibit the apoptosis of nerve cells, reduce the total cerebral ischemia reperfusion injury, which helps protect the brain.
NETL
NSTL
万方数据
维普
