Objective To determine the quality standard of Litong Tea. Methods Folium sennae in Litong Tea was identified by TLC. The content Hesperidin of Pericarpium citri reticulatae was determined by HPLC. Results Folium sennae was identified by TLC; Hesperidin showed a good liner relationship at the range of 0.215-1.720μg, r=0.9997. The average recovery was 100.34%(n=6), The RSD was 1.2%. Conclusion The method is simple, accurate and exclusive with a reproducibility and can be used to control the quality of Litong Tea.