The HPLC method in the determination of Simultaneous Flos Lonicerae in coffee acid and galuteolin
Objective To establish high performance liquid chromatography(HPLC) in the determination of the content of Flos Lonicerae in coffee acid and galuteolin. Methods Taking Agilent C18 column(250mm×4.6mm, 5μm), mobile phase of methanol: water solution of phosphoric acid by 0.1%, according to the gradient elution mode, the flow rate was 1.0ml/min, the injection volume was 15μl, the detection wavelength was 338nm, the column temperature was 25℃ . Results Coffee acid and galuteolin respectively in 8.1-82.0μg/ml, 2.1-42.0μg/ml was with good linear relationship, the linear regression equation was Y=37.71X-265.77(R=0.9997), Y=15.56X+3.98(R=0.9995). Conclusion The method of HPLC method in the determination of coffee acid in Flos Lonicerae Japonicae, galuteolin is simple, and with good separation effect, good repeatability, and it is a better detection of Flos Lonicerae Japonicae quality.
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维普
