首页|156例儿童医院感染性腹泻病原学分析

156例儿童医院感染性腹泻病原学分析

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目的探讨我院医院感染性腹泻(Infectious diarrhea,ID)致病菌的分布,为院内ID防治提供理论依据。方法对2010年1-12月156例院内ID患儿的粪便标本进行常规检查,细菌培养,并采用免疫胶体金标法检测轮状病毒(Rotavirus,RV)抗原;逆转录聚合酶链式反应(RT-PCR)法检测诺如病毒(Norovirus,NV)核酸,星状病毒核酸及肠腺病毒核酸。结果156例患儿细菌感染10例(占6.4%),真菌感染5例(占3.2%),检出RV阳性74例(占47.4%),NV阳性24例(占15.4%),星状病毒阳性2例(1.3%),肠腺病毒阳性6例(3.8%);RV和NV病毒院内感染阳性率男女性别差异无统计学意义(P>0.05),不同年龄组间RV及NV阳性率差异无统计学意义(P<0.05)。RV、NV在不同季节的发病差异均有统计学意义(P<0.05)。结论婴幼儿院内ID病毒多于细菌,病毒中轮状病毒占首位,应根据病原菌分布情况积极采取预防与控制措施,降低医院ID发病率。
Pathogen analysis on 156 cases with infectious diarrhea in Children's Hospital
Objective To study pathogen isolations from infants with infectious diarrhea in Fuzhou Children's Hospital, and to provide basic evidence for reasonable, effective control of infectious diarrhea in hospital. Methods 156 cases with infectious diarrhea from digestive department in Fuzhou Children's Hospital(Jan 2010-Dec 2010) were selected and their faeces samples were cultivated. Rotavirus(RV) were detected using Colloidal Gold method; Norovirus, Astrovirus, intestinal adenovirus, their nuclear acid were detected using RT-PCR method. Results In 156 cases, 10 were found with bacterial infection(6.4%), 5 with fungal infection(3.2%), 74 cases were RV positive(47.4%). 24 were NV postive(15.4%), 2 were Astrovirus positive(1.3%), 6 were intestinal adenovirus positive(3.8%). RV and NV infections obviously had season different and RV infection peaked in October to December, while NV infection peaked in July to September, the difference between seasons had statistical significance(P<0.05). Conclusion Hospital acquired infectious diarrhea was more due to viral infection than bacterial infection, among virus, rotavirus was on the first list, the second was norovirus. Take effective measures to prevent and treat infectious diarrhea in hospital based on our research, so as to reduce the rate of infectious diarrhea in hospital.

InfantInfectious diarrheaPathogenAnalysis

陈凤钦、翁绳凤、卓铃

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350004 福建医科大学教学医院福州儿童医院儿内科

100095 北京老年医院检验科

小儿 感染性腹泻 病原 分析

福建省福州市科技项目

2008-s-81

2013

中国医疗前沿
中国医院协会

中国医疗前沿

影响因子:0.186
ISSN:1673-5552
年,卷(期):2013.(15)
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