目的探讨反义肽核酸(phosphono sense peptide nucleic acid，P-PNA)对肺癌SPC-A-1凋亡作用的影响。方法采用肺癌细胞株(SPC-A-1)培养，待细胞为对数生长期时，传代培养30例标本，分为P-PNA组、脂质体组、空白对照组，每组10例，转染前后用RT-PCR，流式细胞仪检测，分别检测端粒酶活性和细胞凋亡百分率。结果转染后P-PNA的端粒酶活性水平有所下降，ATP的浓度明显降低，空白对照组与脂质体组无明显的变化(P<0.05)。P-PNA组肺癌细胞的凋亡率明显增加，与其他两组比较差异显著(P<0.05)。结论 P-PNA在载体运载下，可以进入SPC-A-1细胞线粒体中，通过影响端粒酶的活性阻碍编码基因的转录，进而影响肺癌细胞的合成并诱导其凋亡。
The expression of antisense P-PNA on apoptosis of SPC-A-1
Objective To investigate the effect of antisense peptide nucleic acid(phosphono sense peptide nucleic acid, P-PNA) on lung cancer SPC-A-1 apoptosis. Methods The lung cancer cells line(SPC-A-1) were cultured, logarithmic growth phase, 30 specimens were taken for subculture. They were divided into the P-PNA group, the liposome group, the blank control group, and 10cases in each group. The RT-PCR, flow cytometry was taken for detection before and after transfection. The percentage of telomerase activity and apoptosis was taken for detection. Results The level of P-PNA after transfection of telomerase activity decreased, the concentration of ATP decreased significantly, the blank control group and liposome group had no significant changes(P<0.05). Group P-PNA lung cancer cell apoptosis rate increased significantly, and there were significant differences compared with the other two groups(P<0.05). Conclusion P-PNA in the carrier vehicle, can enter the mitochondria of SPC-A-1 cells by telomerase activity, impede the transcription of genes encoding, and affect the synthesis of lung cancer cells and induce its apoptosis.
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