The distributive characteristic of genotype of HPA-2 in the volunteer platelet donors
Objective To evaluate the distributive characteristic of genotype of platelet glycoprotein HPA-2 in the volunteer platelet donors. Methods 184 volunteer platelet donors at mobile place were collected, including 44 type A, 44 type B, 55 type O and 41 type AB volunteer platelet donors. HPA-2 polymorphism was tested by polymerase chain reaction-restricted fragment length polymorphism(PCR-RFLP) amplification. Genotyping for HPA-2 was performed by restriction enzyme analysis. Results HPA-2 2a/2a genotype frequency was 0.95, 0.91, 0.85, 0.98 respectively; 2a/2b genotype frequency was 0.05, 0.07, 0.15, 0.02 respectively; 2b/2b genotype was only one and genotype frequency was 0.02 among the 184 volunteer platelet donors with different blood type. Conclusions There is no significant difference of HPA-2 genotype frequency among the volunteer platelet donors of four different blood type.
NETL
NSTL
万方数据
维普
