The effects of EGFR expression and proliferation of breast cancer MCF-7 cells regulated by membrane estrogen
Objective To investigate the proliferation and the possible molecular mechanisms of the breast cancer cell regulated by membrane estrogen continuously. Method The MTT method was used to measure the the growth effects of breast cancer MCF-7 cell regulated by the different concentration of membrane estrogen. The MTT method and RT-PCR method were used respectively to analyze the breast cancer MCF-7 cell growth and the EGFR mRNA expression level regulated by membrane estrogen. Results The sustained action of membrane estrogen on the final concentration from 10-7M to 10-12M can induce breast cancer MCF-7 cell growth. The suramin(G protein-coupled receptor inhibitor) and AG-1478(EGFR inhibitor) can down-regulate the effect of MCF-7 cell growth stimulated by membrane estrogen. Suramin and AG-1478 can down-regulate the EGFR mRNA expression stimulated by membrane estrogen in the MCF-7 cell. Conclusion The continuous estrogen membrane effect can stimulate the proliferation of breast cancer cells, it is through the membrane G protein coupling receptor and EGFR pathway.
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万方数据
维普
