标本保存对实时荧光定量HCV-RNA检测的影响
Impact of the specimen preservation on the detection of serum HCV RNA using FQ-RT-PCR
刘晓 1许琳婧 2孙丽萍1
作者信息
- 1. 116031 大连医科大学附属第二医院钻石湾院区检验科
- 2. 大连市第六人民医院中心实验室
- 折叠
摘要
目的：研究标本保存对实时荧光定量HCV-RNA检测结果的影响。方法收集HCV阳性血清标本42份并平均分成6份分别置于室温、4℃和-20℃保存1d和1周后用荧光定量PCR法检测结果，行统计学分析。结果血清在室温、4℃和-20℃条件下保存1d和1周对HCV RNA的荧光定量检测结果无统计学差异。结论保存温度和保存时间对HCV RNA的定量检测结果无影响。
Abstract
Objective To investigate the impact of various storage conditions of serum samples on HCV RNA detection. Methods Real-time fluorenscent quantitative PCR detected 42 examples have been diagnosed in patients with HCV. Result The results of HCV RNA in serum samples,which stored 1 day and 7 days at room temperature, 4 ℃ and-20℃ , showed no significant differences. Conclusion Save temperature and save time have no effect on test results.
关键词
标本保存/HCV-RNA/FQ-RT-PCRKey words
The preservation of the specimen/Hepatitis C virus RNA/Fluorescence quantitative PCR引用本文复制引用
出版年
2013
NETL
NSTL
维普
万方数据