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标本保存对实时荧光定量HCV-RNA检测的影响

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目的:研究标本保存对实时荧光定量HCV-RNA检测结果的影响。方法收集HCV阳性血清标本42份并平均分成6份分别置于室温、4℃和-20℃保存1d和1周后用荧光定量PCR法检测结果,行统计学分析。结果血清在室温、4℃和-20℃条件下保存1d和1周对HCV RNA的荧光定量检测结果无统计学差异。结论保存温度和保存时间对HCV RNA的定量检测结果无影响。
Impact of the specimen preservation on the detection of serum HCV RNA using FQ-RT-PCR
Objective To investigate the impact of various storage conditions of serum samples on HCV RNA detection. Methods Real-time fluorenscent quantitative PCR detected 42 examples have been diagnosed in patients with HCV. Result The results of HCV RNA in serum samples,which stored 1 day and 7 days at room temperature, 4 ℃ and-20℃ , showed no significant differences. Conclusion Save temperature and save time have no effect on test results.

The preservation of the specimenHepatitis C virus RNAFluorescence quantitative PCR

刘晓、许琳婧、孙丽萍

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116031 大连医科大学附属第二医院钻石湾院区检验科

大连市第六人民医院中心实验室

标本保存 HCV-RNA FQ-RT-PCR

2013

中国医疗前沿
中国医院协会

中国医疗前沿

影响因子:0.186
ISSN:1673-5552
年,卷(期):2013.(22)
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