APCmin/+；Mac-1-/-小鼠模型的构建
Establishment of APCmin/+;Mac-1-/- mice model
雷岩 1章倩倩 1胡曦文 1刘红英 1郑凌云 2顾取良 2何晓东 1王丽京1
作者信息
- 1. 510006 广州，广东药学院血管生物学研究所
- 2. 广东药学院基础学院
- 折叠
摘要
目的：构建APCmin/+；Mac-1-/-小鼠模型。方法将自发肠腺瘤(APCmin/+)模型同β2家族整合素Mac-1缺失的小鼠(Mac-1-/-)进行杂交，剪小鼠尾巴抽提DNA，然后用PCR法鉴定小鼠的基因型。结果将APCmin/+小鼠与Mac-1-/-小鼠杂交3代后，成功构建了APCmin/+；Mac-1-/-小鼠模型。结论将APCmin/+小鼠与Mac-1-/-小鼠杂交3代后成功建立了APCmin/+；Mac-1-/-小鼠模型，进一步扩大种群。
Abstract
Objective To establish the APCmin/+;Mac-1-/- mice. Methods Female PSGL-1 KO mice were mated with male APCmin/+ mice and male progeny carrying the APCmin/+ transgene. Mice were genotyped by PCR. Results After female Mac-1 KO mice were mated with male APCmin/+ mice for three generations, APCmin/+;Mac-1-/- mice were established. Conclusion APCmin/+;Mac-1-/- mice were obtained and propagated through APCmin/+ mice being mated with MAC-1 KO mice.
关键词
结直肠肿瘤/β2整合素/MAC-1/动物模型Key words
Intestinal tumor/β2 integrin/MAC-1/Animal model引用本文复制引用
基金项目
出版年
2013
NETL
NSTL
维普
万方数据