首页|APCmin/+；Mac-1-/-小鼠模型的构建

APCmin/+；Mac-1-/-小鼠模型的构建

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目的：构建APCmin/+；Mac-1-/-小鼠模型。方法将自发肠腺瘤(APCmin/+)模型同β2家族整合素Mac-1缺失的小鼠(Mac-1-/-)进行杂交，剪小鼠尾巴抽提DNA，然后用PCR法鉴定小鼠的基因型。结果将APCmin/+小鼠与Mac-1-/-小鼠杂交3代后，成功构建了APCmin/+；Mac-1-/-小鼠模型。结论将APCmin/+小鼠与Mac-1-/-小鼠杂交3代后成功建立了APCmin/+；Mac-1-/-小鼠模型，进一步扩大种群。
Establishment of APCmin/+;Mac-1-/- mice model
Objective To establish the APCmin/+;Mac-1-/- mice. Methods Female PSGL-1 KO mice were mated with male APCmin/+ mice and male progeny carrying the APCmin/+ transgene. Mice were genotyped by PCR. Results After female Mac-1 KO mice were mated with male APCmin/+ mice for three generations, APCmin/+;Mac-1-/- mice were established. Conclusion APCmin/+;Mac-1-/- mice were obtained and propagated through APCmin/+ mice being mated with MAC-1 KO mice.

Intestinal tumorβ2 integrinMAC-1Animal model

雷岩、章倩倩、胡曦文、刘红英、郑凌云、顾取良、何晓东、王丽京

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510006 广州，广东药学院血管生物学研究所

广东药学院基础学院

结直肠肿瘤 β2整合素 MAC-1 动物模型

31271455、31200896、31100852、31200861B2012181S2012040007658

2013

10.3969/j.issn.1673-5552.2013.23.0016

中国医疗前沿
中国医院协会

中国医疗前沿

影响因子：0.186
ISSN：1673-5552
年,卷(期)：2013.(23)
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