Effect of Granulocyte Colony-Dtimulating Factor to Reconstruction of Micrangium on Experimental Intracerebral Hemorrhage in rat brain tissue
Objective To investigate the effect of granulocyte colony-stimulating factor on expressions of CD34 positive microvascular in rat brain with intracerebral hemorrhage, and to explore its possible neuroprotective effects. Methods Sixty-three Spragne Dawley rats were randomly selected and divided into three groups:sham operation, ICH group and treatment group. There are 21 rats in every group. To utilize stereotaxic apparatus and infuse fresh quantited autologous blood into the right basal ganglia to build up rat experimental ICH model. Rats in the treatment group were administered rhG-CSF(60μg/kg) by intraperitoneal injection post operation 1h,while rats in ICH group and sham operation group were injected with equal volume of isotonic saline solution for injection. Neural function defect of rats were assessment by measurement of Garcia's score method post operation 6h, 24h, 48h, 72h, 7d, 14d, 21d. Then the rats were sacrificed, expression of CD34 positive microvascular around hematoma in rats' brain were analyzed by immunohistochemistry method at the same time point. Results The score of nerve function was obviously increased from 24h in treatment group, and had significant difference compared with ICH group(P <0.05). The expressions of CD34 positive microvascular was little in sham operation group. In ICH group, the expressions of CD34 positive microvascular were increaesed from 6h and peaked at 14d. Compared to ICH group, the expressions of CD34 positive microvascular in treatment group were increased at each time point(P <0.05) and early peaked at 7d. Conclusion G-CSF could promote angiogenesis to exert neuroprotective effects.
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万方数据
维普
