首页|Classical eyeblink conditioning using electrical stimulation of caudal mPFC as conditioned stimulus is dependent on cerebellar interpositus nucleus in guinea pigs

Classical eyeblink conditioning using electrical stimulation of caudal mPFC as conditioned stimulus is dependent on cerebellar interpositus nucleus in guinea pigs

扫码查看
原文链接
  • NETL
  • NSTL
  • 万方数据
  • 维普
Aim:To determine whether electrical stimulation of caudal medial prefrontal cortex (mPFC) as conditioned stimulus (CS) paired with airpuff unconditioned stimulus (US) was sufficient for establishing eyeblink conditioning in guinea pigs,and whether it was dependent on cerebellar interpositus nucleus.Methods:Thirty adult guinea pigs were divided into 3 conditioned groups,and trained on the delay eyeblink conditioning,short-trace eyeblink conditioning,and long-trace eyeblink conditioning paradigms,respectively,in which electrical stimulation of the right caudal mPFC was used as CS and paired with corneal airpuff US.A pseudo conditioned group of another 10 adult guinea pigs was given unpaired caudal mPFC electrical stimulation and the US.Muscimol (1 μg in 1 μL saline) and saline (1 μL) were infused into the cerebellar interpositus nucleus of the animals through the infusion cannula on d 11 and 12,respectively.Results:The 3 eyeblink conditioning paradigms have been successfully established in guinea pigs.The animals acquired the delay and short-trace conditioned responses more rapidly than long-trace conditioned responses.Muscimol infusion into the cerebellar interpositus nucleus markedly impaired the expression of the 3 eyeblink conditioned responses.Conclusion:Electrical stimulation of caudal mPFC is effective CS for establishing eyeblink conditioning in guinea pigs,and it is dependent on the cerebellar interpositus nucleus.

associative learningmemoryeyeblink conditioningmedial prefrontal cortexcerebellar interpositus nucleusmuscimolguinea pig

Guang-yan WU、Juan YAO、Zheng-li FAN、Lang-qian ZHANG、Xuan LI、Chuang-dong ZHAO、Zhen-hua ZHOU、Jian-feng SUI

展开 >

Department of Physiology, College of Basic Medical Sciences, Third Military Medical University, Chongqing 400038, China

Experimental Center of Basic Medicine, College of Basic Medical Sciences, Third Military Medical University, Chongqing 400038, China

Department of Neurosurgery, General Hospital of Ji-nan Command, Ji-nan 250003, China

Department of Neurology, Southwest Hospital,Third Military Medical University, Chongqing 400038, China

展开 >

国家自然科学基金

30771769

2012

10.1038/aps.2012.32

中国药理学报(英文版)
中科院上海药物研究所

中国药理学报(英文版)

CSTPCDCSCDSCI
影响因子：0.926
ISSN：1671-4083
年,卷(期)：2012.33(6)
  • 1
  • 80