首页|山东花生茎腐病病原菌研究

山东花生茎腐病病原菌研究

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  • 国家科技期刊平台
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从山东省多地采集的花生茎腐病株上分离到15个茎腐病菌株，选取6个典型菌株，对病原菌进行形态特征、致病性、生物学特性研究。核糖体DNA-ITS序列分析表明，6个菌株的核糖体DNA-ITS序列同源性高达99％。根据病原菌的形态特征、核糖体DNA-ITS序列同源性比较，确认供试6个菌株为同一种病原菌，与Gen-Bank中可可毛色二孢属（Lasiodiplodia）（Diplodia的同物异名）的同源性高达99％以上。根据6个菌株的形态特征、致病性测定结果及生物学特性，并结合核糖体DNA-ITS序列分析，将山东花生茎腐病的病原鉴定为棉色二孢菌（Diplodia gossypina）。
Research on pathogen of peanut stem rot in Shandong Province
Six representative strains were selected from peanut stem rot plants collected from Shandong Prov-ince.Morphological characteristics,pathogenicity and biological characteristics of 6 representative strains were studied.Ribosomal DNA-ITS sequence analysis showed that the sequence homology of these 6 strains was up to 99%.The tested 6 isolates were confirmed as one species.The homology of DNA-ITS sequences of the 6 strains was up to more than 99% compared with Lasiodiplodia spp (synonyms Diplodia)in GenBank.According to mor-phological characteristics,pathogenicity test and biological characteristics of the 6 tested strains,and combined with the ribosomal rDNA-ITS sequence analysis,the pathogen of peanut stem rot from Shandong Province was identi-fied as Diplodia gossypina.

Peanut stem rotRibosomal DNA-ITSPathogen identification

郭洪参、张悦丽、齐军山、张博、李林、徐作珽、李长松、张广民

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山东农业大学植物保护学院，山东 泰安，271018

山东省农业科学院植物保护研究所/山东省植物病毒重点实验室，山东 济南，250100

花生茎腐病 核糖体DNA-ITS序列 病原鉴定

山东省现代花生产业技术体系

2014

10.7505/j.issn.1007-9084.2014.04.015

中国油料作物学报
中国农业科学院油料作物研究所

中国油料作物学报

CSTPCDCSCD北大核心
影响因子：1.296
ISSN：1007-9084
年,卷(期)：2014.(4)
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