首页|白菜型油菜春化基因BrFLC序列变异分析

白菜型油菜春化基因BrFLC序列变异分析

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  • 国家科技期刊平台
  • NETL
  • NSTL
  • 万方数据
  • 维普
为寻找与冬春性相关的变异,以开发冬春性品种分子辅助标记,采用巢式和半巢式扩增法,对感温性和抗寒性强弱不同的5个白菜型油菜品种的BrFLC基因的第4外显子至第7外显子进行了特异性扩增.除BrFLC5无扩增结果外,其余3个基因均得到扩增.序列比对结果发现,3个BrFLC在第4外显子至第7外显子均存在有不同程度的等位变异,发生的主要等位变异有SNP和Indel两种类型.BrFLC1变异与冬春性相关性不大,与冬春性关系密切的变异主要发生在BrFLC2和BrFLC3上.BrFLC2中检测到1个Indel类型和5个SNP类型变异位点与冬春性有关.BrFLC3中检测到1个Indel类型和16个SNP类型变异位点与冬春性有关.
Sequence variation of vernalization gene BrFLC in turnip rapes (Brassica rapa L.)
To better understand the variations related to winter and spring of Brassica rapa,4 BrFLCs genes from 5 turnip rape cultivars (different in temperature sensitivity and cold hardiness) were investigated by nested and semi nested PCR.Primers were designed between the sequence of exon 4 to exon 7 in BrFLCs.Results showed that BrFLC1,BrFLC2 and BrFLC3 were amplified except BrFLC5.Sequence alignment analysis found some variations in the regions from exon 4 to 7 of the 3 BrFLCs.The main allelic variations were SNPs and Indels.Combining with their temperature sensitivity,variations in BrFLC1 had little correlation to winterness,and those from BrFLC2 and BrFLC3 might have higher correlations.1 Indel and 5 SNPs in BrFLC2,and 1 Indel and 16 SNPs in BrFLC3 might be associated with winterness.

Turnip rape (Brassica rapa L.)Vernalization genesBrFLCsSequence variation

杨刚、孙万仓、王丽萍、曾秀存、刘自刚、李学才、方彦、武军艳、王凯音

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甘肃省油菜工程技术研究中心,甘肃省干旱生境作物重点实验室/甘肃省作物遗传改良与种质创新重点实验室,甘肃农业大学农学院,甘肃兰州,730070

白菜型油菜 春化基因 BrFLC基因 序列变异

国家863计划国家973计划国家公益性行业(农业)科研专项现代农业产业技术体系建设专项国家自然科学基金国家农业科技成果转化项目

2011AA10A1042015CB150206200903002-04CARS-13314603562014G10000317

2015

10.7505/j.issn.1007-9084.2015.02.007

中国油料作物学报
中国农业科学院油料作物研究所

中国油料作物学报

CSTPCDCSCD北大核心
影响因子：1.296
ISSN：1007-9084
年,卷(期)：2015.37(2)
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