Population diversity and antagonistic effect of Trichoderma in soybean fields in Northeast China
Trichoderma is an important soil ecosystem microbial population. In order to clarify the population diversity of Trichoderma in soybean fields in Northeast China,147 soil samples were collected from soybean fields of Liaoning,Jilin and Heilongjiang provinces from 2012 to 2014. A total of 237 strains of Trichoderma were isolated using dilution plate method and soil particles isolation and culture. These strains were identified and grouped into 15 species. The dominant species included Trichoderma harzianum(77 strains,accounting for 32. 49% of isola-tions). Another dominant species was Trichoderma atroviride(49 strains,accounting for 20. 68% of isolations). The numbers of Trichoderma in Liaoning and Jilin were more than that of Heilongjiang Province. T. citrinoviride and T. spirale had been found in Liaoning and Heilongjiang provinces. T. feritile,T. erinaceum,T. parceramosum,T. asperellum and T. strigosum were only found in Liaoning Province. 2 Trichoderma strains,sneft - s57(1)and sneft- l50(2),had higher efficiency in killing nematodes,and stronger antagonism to Fusarium graminearum and Rhi-zoctonia solani.