首页|低温胁迫下白菜型与甘蓝型冬油菜抗寒基因表达差异

低温胁迫下白菜型与甘蓝型冬油菜抗寒基因表达差异

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为了解白菜型和甘蓝型冬油菜抗寒基因的表达差异,以6个抗寒性不同的冬油菜为材料,利用实时荧光定量PCR(qRT-PCR)分析常温对照以及4℃、-4℃和-8℃低温处理5d后BnCOR25、BnICE1和Cu/Zn-SOD三个基因的表达差异.结果表明,常温时白菜型与甘蓝型冬油菜BnCOR25、BnICE1和Cu/Zn-SOD基因未被激活,处于同一水平;随着温度的降低,白菜型冬油菜BnCOR25、BnICE1和Cu/Zn-SOD的相对表达量均上升,且均在-4℃时到达峰值;而甘蓝型冬油菜BnCOR25在4℃时到达峰值,Cu/Zn-SOD在-4℃时到达峰值,BnICE1的相对表达量在低温胁迫过程中始终低于常温水平;-8℃时白菜型与甘蓝型冬油菜各基因表达量均下降.在整个温度降低过程中,始终表现为白菜型冬油菜BnCOR25、BnICE1和Cu/Zn-SOD的相对表达量显著高于甘蓝型冬油菜,抗寒性强的品种高于抗寒性弱的品种.说明,白菜型与甘蓝型冬油菜间抗寒性的较大差异与这3个基因的表达有关.
Expression of cold resistance genes from winter rapeseed of Brassica rapa and B.napus under chilling stress
To understand different cold-tolerance of Brassica rapa and B.napus,3 genes were investigated under chilling stresses at 4℃,-4℃ and-8℃.The genes included BnCOR25 (from B.napus),BnICE1 (from B.napus),and Cu/Zn-SOD (from B.rapa).6 different cold-resistant winter rapeseeds were used as materials and analyzed after 5d chilling treatments and normal control (at 24℃,CK).qRT-PCR results showed that BnCOR25,BnICE1 and Cu/Zn-SOD were not activated and expressed at the same level in both B.rapa and B.napus at 24℃.Under chilling,their relative expressions in B.rapa increased when the temperature decreased,and reached to the peak at-4℃.Among them,BnCOR25 reached the peak at 4℃ and Cu/Zn-SOD at-4℃ in B.napus.BnICE1 expression was always lower than CK.At-8℃,genes expressions of both types were decreased and low.Compared to B.napus,these 3 genes expressions in B.rapa were significantly higher.Compared to cold-sensitive rapeseed,those with higher cold tolerance had higher expression.It indicated that cold-tolerance was related to these 3 genes and two types of rapeseed had their different responses.

Brassica rapaBrassica napusLow-temperatureqRT-PCRGene expression

马骊、孙万仓、刘自刚、方彦、武军艳、李学才、李欣、张淑娟、袁金海

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甘肃省干旱生境作物学重点实验室,甘肃省油菜工程技术研究中心,甘肃省作物遗传改良与种质创新重点实验室,甘肃农业大学农学院,甘肃兰州,730070

白菜型油菜 甘蓝型油菜 低温胁迫 实时荧光定量PCR 基因表达分析

国家现代农业产业技术体系国家自然科学基金国家自然科学基金国家973计划国家863计划国家农业科技成果转化甘肃省自然科学基金

CARS-1331460356315603972015CB1502062011AA10A1042014G10000317145RJZG050

2016

10.7505/j.issn.1007-9084.2016.02.001

中国油料作物学报
中国农业科学院油料作物研究所

中国油料作物学报

CSTPCDCSCD北大核心
影响因子：1.296
ISSN：1007-9084
年,卷(期)：2016.38(2)
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