Hormone dynamics of soybean root knots in response to Meloidogyne incognita infection
Root-knot nematodes (RKN,Meloidogyne spp.) induce the formation of giant cell,namely root -knot,by directing the normal root cell of soybean plant into nematode-feeding cell.To investigate the role of hormone alternation in this process,contents of IAA,ABA,JA-Me,GA and ZR were monitored by enzyme-linked immunoassay (ELISA) method.Results showed that contents of all these hormones were changed during the formation of giant cell in soybean roots.Among the hormones,the contents of JA-Me and ZR were consistently higher than those of control within 28 days after RKN inoculation.IAA content was significantly higher than that of control whereas ABA content was significantly lower than that of control within 5 days after inoculation.After that,IAA content was significantly lower than that of control,meanwhile contents of ABA and GA were consistent to those of control basically,except at 14th day and 21st day after inoculation,in which the contents of ABA and GA were higher than that of control respectively.Analysis of the five endogenous hormonal ratio changes showed that IAA/ABA and JA-Me/ABA ratio changed significantly,ZR/ABA and GA/ABA ratio were stable.These data indicated that IAA,ABA and JA-Me played important roles on formation of root-knot during nematode infection.Our results also assisted in finding the physiological response mechanism of soybean responding to nematode infection,and to provide a new strategy for preventing and treating of root knot nematodes.
国家科技期刊平台
NSTL
万方数据
维普
